ContentsPrefacePart I Gene CloningChapter 1 Introduction to Gene Cloning 1R.R Brief Overview RR.2 Historical Events 3R.3 Significance and Alertness 5Chapter 2 Gene and Genome 72.R Discovery of Hereditary Factor 72.2 Basic Knowledge of Gene 142.3 Sequence Signals Involved with Gene Expression 222.4 About Genomic and Metagenomic Studies 26Chapter 3 Basic Techniques for Experiments with DNA 283.R Enzymatic Tools for Gene Manipulation 283.2 Oligo-nucleotide Tools 343.3 DNA Purification 363.4 Electrophoresis 383.5 In Vitro Synthesis of DNA 403.6 DNA Sequencing 433.7 Macromolecular Hybridization and Blotting 463.8 PCR Techniques 48Chapter 4 Cloning Procedure 534.R Basic Procedure of Cloning 534.2 Selection of Cloning Vectors 544.3 Preparation of Donor DNA 604.4 Joining of Donor and Vector by Ligation 624.5 Selectio n of H o st Cells 624.6 Introduction of Recombinant DNA into Host Cells 644.7 Selection，Screening and Sub-cloning.654.8 Derivative Technology Based on CloNng 69Chapter 5 Application and Impacts 735.1 Initiation of Recombinant DNA Industry 735.2 General Aspects of Application 775.3 Contribution and Impact 83Part II Cell EngineeringChapter 6 Introduction on Cell Engineering 886.1 Brief Overview 886.2 Essentials of Cell Engineering 896.3 Progress Clues 91Chapter 7 Cell Fusion and Hybridization 947.1 General Aspects 947.2 Protoplast Technique 947.3 Basic Methods of Cell Fusion 977.4 Fusant Screening 987.5 Application of Cell Fusion 99Chapter 8 Plant Tissue and Cell Culture 1008.1 Plant Tissue Culture System 1008.2 Gene Transfer to Plants 1088.3 Artificial Seeds and Virus-free Plants 113Chapter 9 Animal Tissue and Cell Culture 1169.1 Establishment of Animal Tissue Culture System 1169.2 Gene Transfer to Animal Cell 1209.3 Animal Hybrids and Hybridoma 1249.4 Approaches of Stem Cell Research 129Chapter 10 Application Examples from Cell Engineering 13210.1 Bioproducts from Cell Engineering 13210.2 Breeding of New Agronomic Varieties 13410.3 Transgenic Animals as Models for Medical Research 138Part III Enzyme TechnologyChapter 11 Fundamental Nature of Enzyme 14111.1 Definition， Basic Nature and Classification of Enzyme 14111.2 Determination of Enzyme Activities 1 4611.3 Factors Affecting Enzyme Activity 15011.4 Resources of Enzyme 1 5711.5 Uses of Enzyme 159Chapter 12 Enzyme Biosynthesis and Products 16312.1 Bio-resources of Enzyme 1 6312.2 About Enzyme Synthesis 17012.3 Enzyme Fermentation and Products R73Chapter 13 Enzyme Recovery&Purification 18113.R Pretreatment of Crude Enzyme Sample 18113.2 Removing of Impurities and Sample Concentration 18213.3 Chromatographic Separation 18413.4 Polishing of Enzyme 19113.5 Rational Formulation of Final Enzyme Products 192Chapter 14 Enzymatic Conversion 19414.1 Enzymatic Conversion 19414.2 Immobilization of Enzyme and Cells 20014.3 Enzymatic conversion in non-aqueous Phase 207Chapter 15 New Approaches of Enzyme Technology 21115.1 Modification on Enzymes 21115.2 Enzyme Carriers for Immobilization 21615.3 Enzyme Inhibitors 21915.4 Ribozyme (Non-protein Enzymes) 222Part IV Industrial FermentationChapter 16 Principles of Fermentation 22516.1 Fermentation Principles 22516.2 Fermentation Products and Application 22816.3 Benefits from the Fermentation Industry 231Chapter 17 Industrial Microorganisms 23517.1 Types and Characters of Industrial Microorganisms 23517.2 Breeding of Industrial Strains 24017.3 Industrial Microbial Growth and Culture Media 24217.4 Inoculation of Industrial Strains 24917.5 Preservation of Industrial Strains 252Chapter 18 Microbial Metabolism and Fermentation Kinetics 25418.1 Metabolism Related to Basic Pathway 25418.2 Metabolic Regulation and Control 26218.3 Stoichiometry of Fermentation Process 26818.4 Fermentation Kinetics 272Chapter 19 Bioreactors and Auxiliary Equipments 28019.1 Structure and Function of Bioreactors 28019.2 Auxiliary Equipment 28519.3 Probes and Sensors 288Chapter 20 Fermentation Down-Stream Technology 29220.1 General Aspects 29220.2 Harvest and Pretreatment 29520.3 Isolation and Purification 30120.4 Product Polishing 30420.5 Waste Treatment and Cleaner Production 306Chapter 21 Progress in Fermentation Engineering 31121.1 Approaches of Up-stream Technology 31121.2 Process Optima.31821.3 Development of Down-streamTechnology 321Appendix 325Terms Translation 325Related Nobel Laureates 336Further Reading 340后记 345
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