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| 內容簡介： |
植物生理学是研究植物生命活动规律、揭示植物生命现象本质的一门学科。植
物生理学是农学、园艺、植物保护、种子科学与工程、设施农业科学与工程、植物科
学与技术、园林等涉农专业的专业基础课程。植物生理学实验是该课程重要的实践性
教学环节，通过实验操作能进一步加深学生对理论知识的理解，提高学生动手实践能
力，将理论和实践有机结合，为解决农业生产实际问题奠定良好的基础。
《植物生理学实验实训综合教程》主要包括植物种子的萌发、水分代谢、矿质元
素缺乏对植物生命活动的影响、植物光合性能的评价、植物碳氮代谢、植物生长物质
的生理效应及其对植物生长发育的影响、植物组织培养、植物逆境生理等内容，一共
9章。本书基本上涵盖了植物生理学所有的章节内容，且充分考虑各章节的重点、难
点，为我们进一步理解和巩固相关理论知识提供了帮助。
本书非常注重理论教学和实践应用的深度结合，由潍坊学院、山东农业工程学
院、潍坊职业学院和山东省农业技术推广中心4家单位合作完成，既有本科高校和高
职院校的教师，也有从事农业技术推广的一线工作人员，他们从不同的专业角度来合
理安排实验内容，通过简单易做的植物实验来验证复杂微观的科学知识，启发大家去
探索植物生长的内在规律，以此提高作物生产潜力。
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| 目錄：
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中文篇
第1章 植物生理学实验基础 3
第2章 植物种子的萌发 14
第3章 植物水分代谢 22
第4章 矿质元素缺乏对植物生命活动的影响 30
第5章 植物光合性能的评价 42
第6章 植物碳氮代谢 59
第7章 植物生长物质的生理效应及其对植物生长发育的影响 72
第8章 植物组织培养 83
第9章 植物逆境生理 95
附 录 115
附录1 相关理论知识巩固与思考115
附录2 化学品标志符号202
附录3 常用酸碱的浓度203
附录4 常用固态酸、碱、盐的物质的量浓度配制参考 203
附录5 常用缓冲液的配制204
附录6 常用酸碱指示剂209
附录7 标准计量单位210
附录8 常用植物生长物质的一些化学特性212
参考文献 213
英文篇
Chapter 1 Experimental Basis of Plant Physiology 217
11 Plant physiology laboratory rules217
12 Classification and preparation methods of chemical reagent218
13 Collection， processing and preservation of experimental material220
14 Basic methods of experimental studies on plant physiology228
Chapter 2 Plant Seed Germination 232
21 Determination of seed germination rate， germination potential and
germination index233
22 Rapid determination of seed viability234
23 Determination of seed vigor236
24 The formation and determination of the amylase activity during seed
germination238
25 Changes in fatty acid content of oil seeds during germination239
26 Changes in amino acid content during seed germination241
Chapter 3 Plant Water Metabolism 243
31 Determination of plant leaf water content， relative water content and
water saturation deficit244
32 Determination of free and bound water content in plant leaves
(Malincic method) 245
33 Determination of plant tissue water potential (Small flow method) 247
34 Determination of plant tissue osmotic potential (Plasma wall
separation method) 249
35 Determination of transpiration rate251
Chapter 4 Effects of Mineral Element Deficiency on Plant Life
Activities 254
41 Element deficiency symptoms in plants (solution culture) 255
42 Determination of nitrate reductase activity259
43 Determination of root activity (α - naphthylamine oxidation method) 261
44 Determination of nitrate nitrogen in plants263
45 Determination of plant phosphorus (molybdenum blue method) 265
Chapter 5 Evaluation of Plant Photosynthetic Performance 268
51 Extraction and physicochemical properties of photosynthetic pigments269
52 Separation of photosynthetic pigments and determination of absorption
spectra271
53 Determination of chloroplast pigment content273
54 Determination of plant leaf photosynthetic rate and gas exchange parameters
276
55 Determination of chlorophyll fluorescence parameters280
56 Determination of ribulose diphosphate carboxylase (RuBPCase)
activity286
57 Determination of phosphoenolpyruvate carboxylase (PEPCase)
activity289
Chapter 6 Plant Carbon and Nitrogen Metabolism 291
61 Determination of sucrose， glucose and fructose content292
62 Determination of free amino acid content294
63 Determination of sucrose synthase and sucrose phosphatase activities296
64 Determination of α - amylase and β - amylase activities298
65 Determination of glutamine synthetase activity302
66 Determination of soluble protein content304
Chapter 7 The Physiological Effects of Plant Growth Substances and
Their Effects on Plant Development 307
71 Effects of auxin on the growth of wheat root and shoot308
72 Effects of gibberellin on the growth of wheat seedlings310
73 Effect of ethephon on the fruit ripening310
74 Abscissive effects of abscisic acid (ABA) on plant petioles311
75 Effects of gibberellin (GA3) and abscisic acid (ABA) on seed
germination313
76 Synthesis of α - amylase in seed induced by gibberellin (GA3) 314
77 Effects of abscisic acid (ABA) on stomatal movement317
78 Determination of phytohormones by liquid chromatography318
Chapter 8 Plant Tissue Culture 320
81 Preparation of culture medium321
82 Sterilization， disinfection and inoculation325
83 Subculture and propagation330
84 Acclimation， transplanting and management of tube seedlings334
Chapter 9 Plant Stress Physiology 336
91 Determination of proline content337
92 Determination of total flavonoid content339
93 Determination of ascorbic acid (AsA， AA) 341
94 Determination of glutathione (GsH) 345
95 Determination of cytoplasmic membrane permeability - conductance
method347
96 Identification of degree of membrance peroxidation - determination of
malondialdehyde content (MDA)349
97 Identification of cell death in plant tissues (Trypan blue staining method) 351
98 Determination of hydrogen peroxide (H2O2) content352
99 Determination of superoxide anion production rate354
910 Determination of superoxide dismutase (SOD) activity356
911 Determination of peroxidase (POD) activity358
912 Determination of catalase (CAT) activity360
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