**部分 显微技术和微生物形态学
PART ONE Microscopic Techniques and Microbial Morphology
实验一 明视野光学显微镜
EXPERIMENT 1 Bright--Field Light Microscope
实验二 暗视野光学显微镜
EXPERIMENT 2 Dark--Field Light Microscope
实验三 单染色法
EXPERIMENT 3 Simple Stain
实验四 革兰染色法
EXPERIMENT 4 Gram''s Stain
实验五 芽孢染色法
EXPERIMENT 5 Spore Stain
实验六 荚膜染色法
EXPERIMENT 6 Capsule Stain
实验七 鞭毛染色法
EXPERIMENT 7 Flagella Stain
实验八 螺旋体的染色和形态观察
EXPERIMENT 8 Spirochetes Morphology
实验九 悬滴法观察细菌的动力
EXPERIMENT 9 Observation of Bacterial Motion with Hanging Drop
实验十 酵母菌的形态观察
EXPERIMENT 10 Yeast Morphology
实验十一 微生物大小的测定
EXPERIMENT 11 Determination of the Size of Microorganisms
实验十二 放线菌、霉菌的菌丝和孢子形态观察
EXPERIMENT 12 Morphology of Mycelia and Spore for Actinomycetes and Molds
实验十三 细菌、放线菌、酵母菌和霉菌的菌落特征观察
EXPERIMENT 13 Colony Characteristics of Bacteria,Actinomycetes,
Yeasts and Molds
第二部分 基本实验操作与培养技术
PART TWO Basic Laboratory and Culture Techniques
实验十四 培养基的配制与灭菌
EXPERIMENT 14 Culture Media Preparation and Sterilization
实验十五接种技术与微生物的分离纯化
EXPERIMENT 15 Culture Transfer Techniques and Isolation of Pure Cultures
实验十六 常用的消毒灭菌方法
EXPERIMENT 16 Commonly Used Sterilization and Disinfection Methods
实验十七 菌种保藏
EXPERIMENT 17 Preservation of Pure Cuhures
实验十八 微生物计数法
EXPERIMENT 18 Counting of Microorganisms
实验十九 细菌的生长曲线测定
EXPERIMENT 19 Bacteria Growth Curve
第三部分 细菌的生理生化反应
PART THREE Biochemical Activities of Bacteria
实验二十 淀粉水解和明胶液化试验
EXERIMENT 20 Hydrolysis of Starch and Protein
实验二十一 糖发酵试验
EXERIMENT 21 Utilization of Carbohydrates
实验二十二 IMViC试验
EXERIMENT 22 IMViC Series
实验二十三 硫化氢产生试验和触酶试验
EXERIMENT 23 Hydrogen sulfide Production and Catalase Test
第四部分 病毒学实验
PART FOUR Experiments for Virology
实验二十四 流感病毒的培养与检测
Experiment 24 Culture and Detection of Influenza Virus
实验二十五 噬菌体分离和效价测定
Experiment 25 Isolation and Titer Detection of Bacteriophage
实验二十六 噬菌体一步生长曲线的测定
Experiment 26 One——Step Growth Curve of Bacteriophage
第五部分 微生物遗传学实验
PART FIVE Experiments for Microbial Genetics
实验二十七 微生物的遗传与变异现象
EXPERIMENT 27 Heredity and Variation of Microorganism
实验二十八 微生物抗药性变异
EXPERIMENT 28 Drug Resistant Variation
实验二十九 组氨酸营养缺陷型菌株的诱变和筛选
EXPREIMENT 29 Mutagenesis and Isolation of his Auxotroph
实验三十 鼠伤寒沙门菌/微粒系统检测化学诱变剂
EXPERIMENT 30 Ames Test:A Bacterial Test System for Detecting Mutagens andCarcinogens
实验三十一抗药质粒接合传递实验
EXPERIMENT 31 Bacterial Conjugation:The Transfer of Antibiotic--Resistant
Plasmids
实验三十二 抗药质粒DNA的分离检测和转化
EXPERIMENT 32 Isolation and Characterization of P1asmid DNA and
Transformation
实验三十三 细菌转导
EXPERIMENT 33 Bacterial Transduction
实验三十四 枯草杆菌原生质体的制备和再生
EXPERIMENT 34 Formation and regeneration ofBacillus subtilis protoplast
实验三十五 利用16s rRNA基因序列进行细菌分类鉴定
EXPERIMENT 35 Bacterial Identification with 16SrRNA Gene
第六部分 免疫学实验
PART SIX Experiments of Immunology
实验三十六 抗原与免疫血清的制备
EXPERIMENT 36 Preparations of Antigen and Antiserum
实验三十七 吞噬细胞功能的测定
EXPERIMENT 37 Assay of the Function of Phagocytes
实验三十八 体液因子的免疫功能测定
EXPERIMENT 38 Assay of Immune Functions of Humoral Factors
实验三十九 体液免疫功能检测(1)
EXPERIMENT 39 Immune Functions by Antibody(1)
实验四十 体液免疫功能检测(2)
EXPERIMENT40 Immune Functions of Antibodies(2)
实验四十一 细胞免疫功能检测(1)——体外实验
EXPERIMENT 41 Immune Function by Cellular Immunity(1)——in Vitro
实验四十二 细胞免疫功能检测(2)——细胞因子的检测
EXPERIMENT 42 Immune Function by Cellular Immunity(2)
——Detection of Cytokines Levels
实验四十三 细胞免疫功能检测(3)——体内实验
EXPERIMENT 43 Immune Function by Cellular Immunity(3)——in Vivo
第七部分 药学微生物学实验
PART SEVEN General Mierobial Experiments in Pharmaeeutics
实验四十四 药物的体外抗菌试验
EXPERIMENT 44 Determination of Antimicrobial Activityin Vitro
实验四十五 抗生素效价测定
Experiment 45 Assay of Antibiotic Potency
实验四十六 药物的无菌检查法
EXPERIMENT 46 Sterility Test of Drugs
实验四十七 药物的微生物限度检查法
EXPERIMENT 47 Microbial Quality and Microbial limit Tests 0f
Pharmaceutical Preparations
第八部分 综合性实验
PART EIGHT Integrated Experiments
实验四十八土壤中抗生素产生菌的分离、鉴定和抗菌活性测定
EXPERIMENT 48 Isolation and Identification of Antibiotic-Producing
Actinomycetes from Soil,and the Determination of its
Antimicrobial activity
实验四十九 重组人干扰素-α工程菌的构建、培养和诱导表达
EXPERIMENT 49 Construction Expression and Cuhivation of Recombinant
E.coli Expressing INF-α
实验五十 产淀粉酶枯草杆菌的选育
EXPERIMENT 50 Screening ofBacillus subtilis with High Produetivitv of
Amylase
实验五十一 产β-内酰胺酶抗药细菌的分离和鉴定
EXPERIMENT 51 Isolation and Identification of β-lactamase producing
Staphylococcus aureus
实验五十二污水中大肠埃希菌噬菌体的分离、培养和效价测定
EXPERIMENT 52 Isolation,Cultivation and Titration ofEscherichia coli phage from
Sewage
第九部分 设计性实验
PART NINE Designed Experiments
实验五十三 药品的微生物学质量标准的建立
EXPERIMENT 53 Microbial Quality Control of Pharmaceuticals
实验五十四 营养缺陷型菌株的筛选和鉴定
EXPERIMENT 54 Isolation and Identification of Auxotroph
实验五十五 葡萄中酵母菌的分离、培养和鉴定
EXPERIMENT 55 Isolation,Culture and Identification 0f Yeast in Grapes
实验五十六 菌种的复壮
EXPERIMENT 56 Strain Reiuvenation
实验五十七 基因工程菌种子库的构建
EXPERIMENT 57 Establishmerit of Seed Bank of Genetic Engineered Bacteria
实验五十八 环境中微生物的检测
EXPERIMENT 58 Detection of Environmental Microorganisms
实验五十九 利用BIOLOG自动分析系统分离鉴定人体正常菌群
EXPERIMENT 59 Isolation and Identification of Normal Flora in Human Body
Using BIOLOG Automatic Analysis System
实验六十 药物对流感病毒的抑制作用
EXPERIMENT 60 In Vitro Inhibition of Influenza Virus by Antiviral drugs
实验六十一 实验药物对机体的免疫调节作用
EXPERIMENT 61 Regulatory Effects of Pharmaceuticals on Immunity
实验六十二 抗原抗体反应在伤寒疫苗制备中的应用
EXPERIMENT 62 Application of Antigen—Antibody Reaction in Preparation of
Typhoid Vaccine
附录
Ⅰ.常用无菌玻璃仪器的准备和废品处理
Ⅱ.教学用菌种
Ⅲ.常用培养基
Ⅳ.染色液的配制
Ⅴ.试剂及缓冲液配制
APPENDIX
Ⅰ.Sterilization of Glass Apparatus and Treatment of Waste
Ⅱ.Microorganisms
Ⅲ.Culture Media
Ⅳ.Staining Solutions
Ⅴ.Solutions and Buffers
专业名词表
Words and Vocabulary
参考文献(References)