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The present book is the second version revised from CellEngineering Second Edition, and was selected as a nationalteaching material for Chinese higher education in the 11t" Five- Year Plan. 《Cell EngineeringSecond Edition》包括：comprises three parts: fundamentals of cellengineering, plant cell engineering, and animal cell engineering.Every effort has been made to comprehensively, systematicallyand concisely present the principles as well as techniques inrelation to cell engineering. It is not only suitable as a text bookfor undergraduates majoring in bioengineering, biotechnology,medicine and pharmacy, agriculture etc, but also suitable as areference book for cell engineering researchers
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Preface
Part 1 Fundamentals of Cell Engineering
Chapter 1 Introduction to Cell Engineering
1.1 Bioengineering
1.2 Cell Engineering
Chapter 2 Cell Engineering Basics
2.1 Composition of Cells
2.2 Cell Cycle Division
2.3 Cell Apoptosis Cytoclasis
2 4 Differentiation Dedifferentiation
2.5 Tissue Organ
2.6 Reproduction Development
Part 2 Plant Cell Engineering
Chapter 3 Plant Tissue Organ Culture
3.1 Plant Tissue Culture
3.2 Plant Embryo Culture
3.3 Hairy Root Culture
Chapter 4 Artificial Seeds Virus-Free of Plants
4.1 Artificial Seeds
4.2 Virus Elimination of Plants
Chapter 5 Plant Cell Culture Preparation of Secondary Metabolites
5.1 Plant Cell Culture and Its Characteristics
5.2 Plant Cell Culture Techniques
5.3 Production of Secondary Metabolites by Plant Cell Culture
Chapter 6 Protoplast Culture Mutagenesis
6.1 Protoplast Culture
6.2 Protoplast Mutagenesis
Chapter 7 Cell Fusion Somatic Cell Hybridization
7 I Cell Fusion
7.2 Somatic Hybridization
Chapter 8 Polyploid and Haploid Plants
8.1 Chromosome Engineering
8.2 Polyploid Plants
8.3 Haploids and Homozygous Diploid Plants
Chapter 9 In Vitro Fertilization
9.1 Plant in Vitro Fertilization
9.2 In Vitro Pollination
9.3 In Vitro Fertilization
Chapter 10 Transgenic Techniques
10.1 Transgenic Plants
10.2 Genetically Modified Plants
10.3 Biopharmaceutical Transgenic Plants
10.4 Safety of Transgenic Plants
Part 3 Animal Cell Engineering
Chapter 11 Animal Cell Culture Preparation and Expression of Medicinal Proteins
11.1 Animal Cell Culture
11.2 Characteristics of Animal Cell Culture
11.3 Culture Tools and Conditions
11.4 Growth of in Vitro Cultured Cells
11.5 Cell LineStrain and Preservation
11.6 Small-Scale Animal Cell Culture Methods
11.7 Large-Scale Animal Cell Culture
11.8 Animal Cell Culture Bioreactors
11.9 Factors of Large-Scale Animal Cell Culture
11.10 Preparation of Pharmaceutical Proteins with Animal Cells
Chapter 12 Hybridoma Technology and Monoclonal Antibody
12.1 Hybridoma Technology
12.2 Monoclonal Antibody
12.3 Humanized Monoclonal Antibody
Chapter 13 In Vitro Fertilization Animals Cloned by Nuclear Transfer
13.1 Embryo Technique
13.2 IVF Animals
13.3 Test-Tube Baby
13.4 Animals Cloned by Nuclear Transfer
13.5 Cryopreservation Technique
13.6 Mammalian Sex Control
Chapter 14 Polyploid and Transgenic Animals
14.1 Polyploid Animals
14.2 Transgenic Animals
Chapter 15 Stem Cells
15.1 Characteristics of Stem Cells
15.2 Embryonic Stem Cells
15.3 Adult Stem Cells
Chapter 16 Tissue Engineering
16.1 Tissue Engineering and Its Three Elements
16.2 Technical Routes of Tissue Engineering
16.3 Tissue Engineering Bioreactors
16.4 Tissue Engineering Products
Index
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